ALTERATIONS OF GENE EXPRESSION OF SR Ca2+_ TRANSFER
PROTEINS IN INFANTS WITH CONGESTIVE HEART FAILURE
Yin W, Zheng X-Q, Deng
S-Z
Department of
Cardiology, Wuhan Children��s Hospital, Wuhan, China
Objective: To investigate changes of mRNA expression levels of
calcium-adenosin triphosphatase and ryanodine receptor in infants with
congestive heart failure (CHF); To explore the molecular mechanism of CHF
in infants.
Methods: By reverse transcription-polymerase chain reaction
(RT-PCR), we measured expression levels of SR Ca2+-ATPase
mRNA and RyR mRNA in the right atrial tissue from fourty eight children (M
28, F20) with congenital heart defect betreen the ages of 6 months and 3
years. They were divided into three groups with CHF (group A: moderate to
severe CHF; group B: mild CHF; group C: No CHF).
Results: The level of SR Ca2+-ATPase mRNA
in group A was significantly lower than group B and C (P<0.01). The
level in group B was mildly higher than group C, but without statistical
significance (P>0.05); The level of SR RyR mRNA in group A was
significantly lower than group B (P<0.05) and group B also
siginificantly lower than group C (P<0.05). Meanwhile, the RyR mRNA
expression level was linear related negatively with the CHF severity (Ross
mark) ( r = -0.36, P<0.05).
Conclusion: The down-regulation of cardiac SR Ca2+-ATPase
mRNA and RyR mRNA expression maybe the key fectors of development of
CHF in infants.