LOW-MOLECULAR-WEIGHT HEPARINS AND TRIAMCINOLONE ACETONIDE INHIBIT
PROLIFERATION AND MCP-1 EXPRESSION AND SECRETION IN RAT GLOMERULAR
MESANGIAL CELLS BY NF-kappa B
Wu Xiao-Chuan, Yi Zhu-Wen, Xiao
Jian-Wu, He Xiao-Jie
Laboratory of Pediatric Nephrology, The
Second Hospital of Xiang Ya School of Medicine, Central South
University, Changsha, China
Objective: In order to
investigate the mechanism that Low-Molecular-Weight Heparins (LMWH) and
Triamcinolone Acetonide (TA) inhibit proliferation of glomerular mesangial
cells (GMCs).
Methods: Rat GMCs line was cultured in vitro,
Lipopolysaccharide (LPS), LPS and LMWH, LPS and TA were added into the
culture medium, named as LPS group, LMWH group, TA group, respectively.
GMCs cultured without any drugs served as control group, named as GMCs
group. GMCs proliferative rate was detected by MTT, MCP-1 concentration was
determined by ELISA, MCP-1 and NF-kappa B expression were checked by
immunohistochemistry.
Results: Both in
LMWH group and TA group, the GMCs proliferative rate were obviously lower
than that either in LPS group or in GMCs group (p<0.01). The MCP-1
expression of GMCs in LMWH group was obviously lower than that in any of
the other groups (p<0.01). The MCP-1 expression of GMCs both in TA group
and GMCs group were obviously lower than that in LPS group (p<0.01),
while it had no difference between in these two groups (p>0.05).
Contrast to LPS group, the MCP-1 concentration in culture supernatant were
significantly lower both in LMWH group and TA group (p<0.01), while it
had no difference compared with that in GMCs group (p>0.05). The NF-kappa
B expression in GMCs both in LMWH group and TA group were significantly
lower than that in LPS group (p<0.01), while in LPS group, it was
obviously higher than that in GMCs group (p<0.01), however, in LMWH
group, TA group and GMCs group, it had no significant difference between
any two of them (p>0.05).
Conclusions: It
suggests that LMWH and TA can obviously inhibit proliferation
of GMCs, downregulate the abnormal expression and secretion of MCP-1,
inhibit activation of NF-kappa B, but it has no effect on the normal
activation of NF-kappa B. Perhaps, LMWH and TA inhibit proliferation of
GMCs and MCP-1 expression in GMCs via reducing activation of NF-kappa B.