THE PRIMARY
IMMUNE RESPONSE PATTERN OF THE TWO GROUP MICE WHICH HAD BEEN IMMUNIZED WITH
A SINGLE DOSE 500 MG OR 1000 MG OF E PROTEIN OF DENGUE VIRUS
*Candra
Bumi, *Fedik A Rantam, **Soegeng Soegijanto, **Nur Hidayah
*Tropical
Disease Center, Surabaya
**Child
Health Department of Dr.Soetomo Hospital Surabaya
Objective: To express the IgM and
the IgG antibody level of primary immune response in animal model after
injected E protein of Dengue virus infections.
Method: This study used the
strains of BALB/C of 40 mice (mus musculus) that had never been infected
with dengue virus. The mice were divided three groups. The first group
consisted of 10 mice that were immunized with PBS as control group. The
second and the third group consisted of 15 mice respectively. The second
group was immunized with E protein fraction 500-mg dosage per each whereas
the third group was immunized with E protein fraction 1000-mg dosage per
each. The serum of mice were collected for 4,7,10,15,22 and 30 days after
immunized and analyzed with ELISA for measuring content of immunoglobulin
produced. The fifth day the blood of mice was performed flowcytometri
analysis to determine cellular immunity.
Result: There were differences
significantly between IgM level and IgG level as well as among the
immunoglobulin level of IgG, IgG1, IgG2a and IgG2b both in the 500 mg and
the 1000 mg dosage (p < 0.005). There increased 3.7% and 4.9% of CD4 and
CD8 respectively of cellular immunity.
Conclusion: The primary immune
response in the study showed there were differences significantly between
IgM level and IgG level as well as among the subtype of immunoglobulins
both in the 500 mg dosage and the 1000 mg dosage. There also increased CD4
and CD8 of cellular immune.
Key word: Primary immune
response, E protein of Dengue virus, Immunoglobulin