THE EFFECTS OF OXIDATIVE STRESS DURATION TIME ON THE ACTIVITY OF
ANTIOXIDATIVE ENZYMES: GLUTATHIONE PEROXIDASE (GPX) AND SUPEROXIDE
DISMUTASE (SOD)
Jawniak R, Frelek-Karska M,
Kulik-Rechberger B
Department of
Paediatrics, Medical University School of Lublin
Aerobes have formed
defence mechanisms in response to oxidative stress, which is the cause of
many diseases. Those mechanisms include antioxidative enzymes: GPX and SOD.
Objective: To evaluate GPX and SOD activity depending on oxidative stress duration
time in infants and babies.
Methods: The study group consisted of 74 babies aged 1-23 mths (mean 7.08��4.09
mths) hospitalised for acute pneumonia and/or bronchitis. The babies were
divided into two subgroups: subgroup A: 54 (72.97%)-disease duration time
from the onset to diagnosis 1-7 days, subgroup B: 20 (27.03%)-8 to 43 days
from the onset to the examination.
Blood for examinations was drawn from the peripheral vein. SOD
activity was determined by colorimetry with the use of Ransod kit, GPX
activity with Ransel kit. The results were analysed statistically by
nonparametric tests.
Results: The results concerning the effects of the acute stage of the
disease on GPX and SOD activity are presented in the table.
|
Parameters
|
|
Group A
|
Group B
|
p
|
|
SOD
(U/ml)
|
Mean �� SD
|
86*�� 23,55
|
100,74*��16,34
|
p=0,026
|
|
GPX
(U/ml)
|
Mean �� SD
|
3,55*��1,33
|
5,35*��3,79
|
p=0,004
|
Conclusions: Prolonged oxidative stress caused by infection increases
significantly antioxidative enzymes activity.