THE
STATES OF FUNCTION OF T CELLS AND ROLES OF CYCLOSPRINE A IN CHILDREN WITH
HENOCH-SCHONLEIN PURPURA
LI Q, Yang X-Q, LI Y-B, Wang L-J
Children��s Hospital, Chongqing University of Medical
Science, Chongqing, China
Objective: To observe the states of function of T cells and
to investigate the roles of Cyclosporin A (CsA) in T cells in the children
with Henoch-Schonlein purpura (HSP) in the acute phase.
Methods: The cells count of CD30
expression in Peripheral blood mononuclear cell (PBMC) were determined by
flow cytometry. IgA, IgG, IgM, IgE��Ig
subsets and cytokines were detected by radial immunodiffusion and ELISA.
Results: The serum IgA and IgE
levels, and IgG subsets, IgE, IL-4 and IL-5 levels in the supernatants of
cultured PBMC of HSP patients were increased significantly in contrast to
the normal controls. IL-2 and IFN-��were
decreased. The raised of IL-5 and IL-4 in the supernatants of cultured PBMC
of HSP patients were related
to the increased of IgA����=0.63��and IgE����=0.87��
in serum. The leveals of high TH2 cytokines such as IL-4,IL-5 and IL-6could
be affected by CsA in vitro. The TH1 cytokines further were inhibited.
Conclusions: the dysfunction of T
cell were related to the pathogenesis of HSP patients. The inhibition of
CsA on T cells function of HSP patients were showed. To inhibited TH1
function mainly.