MOLECULAR EPIDEMIOLOGY OF ROTAVIRUS GASTROENTERITIS OF
YOUNG CHILDREN IN CHONGQING
Wang NS, Zhang J, Deng B, Zhu J
Children��s Hospital, Chongqing University
of Medical Sciences, Chongqing, China
Objective: To study the molecular epidemiology
of rotavirus gastroenteritis of young children in Chongqing from 1998 to
2000.
Methods: RNA of rotavirus extracted from
stool samples and RT-PCR and nucleotide sequencing used for gene typing of
G serotype rotavirus.
Results:
130 fecal specimens of diahrrea were detected in the rotavirus from
1998-1999. Fifty speciments (38.46%) were rotavirus-positive. Serotype G1
was predominant and accounted for 88%(44/50).Serotype G3
accounted for 8%(4/50), coinfection of G1and G 3 were
4%(2/50). However during the epidemic season from1999-2000, 38 (33.9%) samples
were rotavirus -positive from 112 stool samples. Serotype G3 was
predominant, up to 78.95%(30/38), only 13.16%(5/38) was serotype G1.
G1 and G3 mixture were7.89%(3/38).
Conclusions:
1. It is demonstrated that serotype G1 and G 3 seem
to be the predominant serotypes of rotavirus in Chongqing. 2. This study is
effective for development and evaluation of the rotavirus vaccine in this
area.