promoter
SEQUENCE analysis of CYP21 AND CYP21P GENE
Wang XM, Han B, Gu XF
Department of Research Center,
XinHua Hospital, Shanghai Second Medical University, Shanghai, China
Objective�� To analyze promoter
sequence of CYP21 and CYP21P gene in 27 patients with congenital adrenal
hyperplasia(CAH) and in normal control (50 Chinese and 6 European).
Methods�� A 1.5 kb fragment of CYP21
genes containing -770bp to -1bp promoter sequence was amplified by specific
PCR primer, the PCR product was studied by DNA sequencing, restriction
fragment length polymorphism (RFLP) using restriction endonuclease Bst�� and Msl��.
Results�� According to Genebank number of M12792 M23280(CYP21 gene) and M12793(CYP21P gene), four nucleotide variations were detected in the promoter of CYP21
gene: -352T to G,-349insG,-332insG,-281insT and three
nucleotide variations were found in the promoter of CYP21P gene: -352T��G,-328insG and -279insG.
All variations were confirmed in CAH group and control
group (Chinese
and European) in all person.
Conclusions�� The nucleotide
variations that we found in promoter of CYP21 gene and CYP21P gene should
be normal sequence.