A STUDY ON BACTERIAL ETIOLOGY OF CHILDHOOD PNEUMONIA
BY SEROLOGICAL ANTIBODIES DETECTION AND CULTURE IN HEFEI, CHINA
Wang YT1, Yang YH2, Hu YW1, Wang YJ2,
Peltola H3, Leboulleux D4, Leinonen M5
1 The First Hospital Affiliated to Anhui Medical
University, Hefei, China
2 Beijing Children��s Hospital, Beijing, China
3 HUCH Hospital for Children and Adolescents,
Helsinki, Finland
4 Aventis Pasteur, Lyon, France
5 National Public Health Institute, Oulu, Finland
Objective: To disclose the incidence of
Streptococus pneumoniae (SP), Haemophilus influenzae (HI), Branhamella
catarrhalis (BC), Chlamydia pneumonia (CP) and Mycoplasma pneumoniae (MP)
in pediatric community-acquired pneumonia in Hefei, China.
Methods: The Enzyme immunoassay (EIA)
was performed to detect anti-non-capsulated HI antibody (Hi-Ab), anti-HI
type-b (Hib) capsular polysaccharide antibody (HibO-Ab) and whole cell
antibody (HibW-Ab), anti-SP pneumolysin antibody (Ply-Ab) and
C-polysaccharide antibody (CPs-Ab), circulating pneumococcal immune complexes
(IC) Ply-Ab (PlyIC), CPS-Ab (CPsIC) and pneumococcal surface protein A
antibody (PsaIC), anti-BC antibody (B.cat-Ab) and anti-CP antibody
(CP-IgG,IgM) from paired sera in 53 cases of hospital-treated children with
pneumonia. At the same time, blood cultures were done in 53 cases and anti-MP
antibodies (Mp-IgG,IgM) were detected in 42 cases by EIA as well.
Results: The evidence of
bacterial infection was obtained in 32 (60.4%) cases of the 53 pneumonia
children, of which SP was the most common agent (11 cases; 20.8%), followed
by CP (10; 18.9%), HI (9; 17.0%; of which Hib was 7 cases), MP
(7/42;16.7%), BC (5; 9.4%) by serologic assays. Staphylococcus aureus,
Klebsiella pneumoniae, Group D streptococcus were cultured in only one case
(1.9%) respectively. More than one specific bacterial co-infection was
found in 16 patients (30.2%).
Conclusion: Bacterial pneumonia
in children is still very common in Hefei, of which SP was the most common
agent.