RAT
BONE MARROW STROMAL CELLS DIFFERENTIATE INTO ASTROCYTES AND NEURONS
COCULTURED WITH ASTROCYTES
Jia YJ, Yang YJ, Song YZ
Xiangya
Hospital, Central South University, Changsha, China
Objective: Bone marrow stromal cells (MSCs) are able to express gene
products typical of neuron and glia after infused into brain. In this
paper, we investigated the effects of direct coculture MSCs with
astrocytes.
Method: MSCs from adult rats were labeled
with bis-benmiadine for 24h, and
cocultured with astrocytes for 21d. The positive percentages of glial fibrillary
acidic protein (GFAP)
and neuron-specific
enolase (NSE), 200-kilodalton neurofilament (NF) expression, were measured by
immuno-cytochemistry ABC-cy3 staining by fluorescence microscope.
Results: After cocultured for 1d to 3d, the
positive percentages of GFAP, NSE and NF expression were <1%. For 7d, the positive percentages of GFAP expression was 8.6%��2.4%, and NSE, NF were 3.8%��2.5% and 4.3%��1.9%, respectively. For
14d, the positive percentages of GFAP expression was increased to 25.0%��9.1%, and NSE, NF were increased slightly,
which 4.5%��1.3% and 5.7%��1.8%, respectively. For 21d, GFAP expression was decreased to 22.9%��7.6%, and the positive
percentages of NSE, NF
were 5.5%��3.6% and 5.3%��2.8%, respectively.
Conclusion: The results suggest that MSCs
may differentiate into
astrocytes and neurons cocultured with astrocytes. It is useful vehicles
for both cell and gene therapy for a variety of diseases of the central
nervous system.