THE RELATIONSHIP BETWEEN HLA-DM AND
TYPE 1 DIABETES IN CHINESE
Sang YM, Yan C, Ni
GC, Zhu C, Hu YM
Beijing Children's Hospital, Capital
University of Medicine Science, China
Objective: To study the relationship between human
leucocyte antigen (HLA)-DMA, DMB alleles and the susceptibility of type I
diabetes in Chinese.
Methods: Genomic DNA were prepared from
peripheral blood by standard method, then polymerase chain reaction and dot
blot hybridization techniques were used to classify 4 DMA and 5 DMB alleles.
Results: DMA*0103, DMB*0103 alleles were
significantly increased in the patients, (50% vs. 8% P<0.01; 42.9% vs.
21.8% P<0.01 respectively), while DMA*0102(10.8% vs. 42%, P<0.01), DMB*0101
(48.6% vs. 73.6%, P<0.01) alleles were significantly less frequent in
patients with type 1 diabetes. DMA*0101/0102 (10.8% vs. 37%, P<0.01), DMB0101/0101
(7.1% vs. 46%, P<0.01) genotype were significantly increased in controls,
while DMA*0101/0103 (50% vs. 8%) genotype were increased in the patients
with the difference also being significant.
Conclusions: Our results showed that DMA*0103,
DMB*0103 alleles confer susceptibility while DMA*0102, DMB*0101 alleles
confer protection to type 1 diabetes. At the same time, DMA*0101/0102, DMB0101/0101
are protective genotypes while DMA*0101/0103 are susceptive genotype to
type 1 diabetes in Chinese.