0761

STUDY ON ETIOLOGICAL DIAGNOSIS OF CHLAMYDIA PNEUMONIAE PNEUMONIA

Cheng H-J, Zhang Y-N, Lu J-R, Liu G-Y

First Hospital, Jilin University, Changchun, China

Objective: To access the morbidity of chlamydia pneumoniae (C.pn) penumonia, evaluate two methods (nPCR and MIF) in detecting the infection of C.pn.

Methods: Both nPCR and MIF were used to detect C.pn in 300 children with pneumonia and 130 healthy children. The direct sequening of products of 2 samples positive to nPCR were analyzed.

Results: Of 300 cases, 16 were positive to nPCR while 8 were positive to MIF under 3 years old children (including 3 years old), the positive rate were 13.3% (16/20) and 6.7% (8/120); 26 were positive to n PCR while 23 were positive to MIF over 3 years old children, the positive rate were 14.4% (26/180) and 12.8%, 30 cases were positive to both nPCR and MIF, the positive rate was 10.0% (30/300). 130 healthy samples were all negative to nPCR whilt 24 were positive to MIF (IgG titer: 1:16~128), the positive rate was 18.4% (24/130). The direct sequening of 2 samples products with nPCR were quite same as C. Pneumoniae (CWL-29).

Conclusion: C.pn is an important pathogenic organism of respiratory tract infection of children. nPCR is sensitive,specific and rapid . MIF applicated with nPCR could increase the sensitivity and specificity of diagnosis of C.pn infection.