Mild hypothermia prevents and cures
systemic inflammatory response syndrome
HU Haofu, XIE Qilian, AN Huibo.
Intensive Care Unit, The Children's Hospital of
Hebei Province, Shijiazhuang, China
Objective: To explore
the inhibiting effect of mild hypothermia on systemic inflammatory response
syndrome (SIRS), thereby to prevent the occurrence of multiple organ
dysfunction syndrome (MODS).
Methods: Ninety-six
mice were randomly divided into four groups: the normal control, lipopolysaccharide
(LPS), mild hypothermia and temperature preservation. By using the
radio-immunity technique, the plasma levels of tumor necrosis factor-�� (TNF-��)in the 4 groups of mice were
measured after 1,2 and 4 hours of injection with LPS into mice. Livers and
lungs of the mice were collected 4 hours after the injection of LPS for
testing the expression of intercellular adhesion molecule-1 (ICAM-1) in
vascular endotheliocytes.
Results: The contents
of TNF-�� in mild
hypothermia group after 1,2 and 4 hours of LPS injections [(2.0��0.3), (2.1��0.4) and (2.5��0.5) ��g/L, respectively] were significantly
lower than those in LPS group [(4.1��0.4), (4.1��0.6) and (3.3��0.5��g/L, respectively) (p<0.001).While there was no significant
difference between LPS and temperature preservation groups after 1 and 2
hours of the LPS injections [(2.0��0.3) and (2.1��0.4) ��g/L vs. (4.0��0.3) and (3.8��0.4) ��g/L, respectively. P<0.01 and <0.05], but no difference
after 4 hours [(2.5��0.5) vs. (2.6��0.5) ��g/L, P>0.05]. Immunohistochemistry staining showed that the
expression of ICAM-1 surrounding the vascular endotheliocyte of the liver
and lung was weakly positive in the mild hypothermia group, but strongly
positive in the LPS group and temperature preservation group.
Conclusion: Mild hypothermia revealed a
function of inhibiting SIRS. It suggested that mild hypothermia may be a
new method for preventing and curing SIRS.
Key words:
Hypothermia, induced; Sepsis syndrome; Tumor necrosis factor; Intercellular
adhesion molecule-1; Mice