STUDY ON THE EFFECT-TIME OF
INFLAMMATORY CYTOKINES IN POSTASPHYXIAL RENAL INJURY IN NEONATAL RATS
Dong W-B, Tang Z-H, Zhai X-S
Department of
Pediatrics, Affiliated Hospital of LuZhou Medical College, Luzhou, Sichuan
Province, China
Objective: To determine the effect-time of
inflammatory cytokines in postasphyxial renal injury in neonatal rats.
Methods: 45
neonatal Wistar rats of 7-10 days were randomly devided into 4 groups: the
control (n=13), 2 hour (h) of reoxygenation (n=10), 24h of reoxygenation
(n=11) and 48h of reoxygenation (n=10) after asphyxia for 30 minutes. The
experiment group was established by normobaric asphyxia, left renal
coefficient (LRC) were calculated, leukocyte infiltration and the scores of
injured tubules were evaluated under light microscopy. Radioimmunoassay
(RIA) was used to measure the contents of interleukin-1 (IL-1) and
interleukin-8 (IL-8) in renal tissue.
Results: Compared
with the control, leukocyte count, LRC, the scores of tubules, IL-1 and
IL-8 increased after 2 h and get to the peak at 24 h of reoxygenation.
Conclusion: It is suggested that release of inflammatory cytokines may play an importmant
role in thedevelopment of postasphyxial renal injury, and its maximal
effect is at 24h of reoxygenation after asphyxia.