QUANTITATIVE ANALYSIS OF ADHESION
MOLECULE CD11B SURFACE EXPRESSION ON NEUTROPHILS FROM NEONATES AND ADULTS
Cui YB1, Du LZ2, Tang YM2,
Chen FY1
1 Women and Children��s Hospital,
Ningbo, China
2 The Children��s Hospital, Medical
School of Zhejiang University, Hangzhou, China
Objective: To investigate the
normal values of adhesion molecule CD11b surface expression on neutrophils
in neonates and the changes of expression level during development.
Methods:
The adhesion molecules CD11b antigen expression
on term Neutrophils from the cord blood after uncomplicated vagina
deliveries (VD, n=25) and elective Caesarean sections (ECS, n=20) and from
the venous blood (n=15) of infants were measured by quantitative flow
cytometry with unfractionated whole blood method. Normal healthy adults
(n=10) were selected as controls.
Results: The mean fluorescence intensity (MFI) of CD11b antigen were
similar among three groups of VD, ECS cord blood and adult venous one (all
P>0.05). The venous blood
in term neonates showed much higher CD11b MFI than two cord blood samples
and also adult group (all P<0.05).
There was no significant difference in positive percentage of CD11b
antigen (CD11b+%) on resting neutrophils among four groups (all P>0.05).
Conclusions: Normal or enhanced adhesion function on resting neutrophils in
term infants may have been established. Mode of delivery may not influence
neutrophil CD11b expression in term neonates.