ASSOCIATION BETWEEN GENETIC POLYMORPHISMS OF THE
INTERLEUKIN-10 GENE PROMOTER AND CLINICAL PHENOTYPES IN CHILDHOOD ASTHMA IN
CHINA
Zhang JL1, Chen H2, Hu LP3,
Fu J1, Zhang HQ1, Chen YZ1
1 Capital Institute of
Pediatrics, Beijing, China
2 Chinese Academy of
Medical Sciences, Beijing, China
3 Chinese Military Academy of Medical
Sciences, Beijing, China
Objective: To determine whether genetic polymorphisms in the promoter
region of the interleukin-10 (IL-10) gene affected clinical phenotypes and
asthma susceptibility in childhood asthma in china.
Methods: One hundred and
sixty-eight unrelated childhood asthma cases and 70 normal control children
were studied. The total serum IgE��prick skin test (PST) to
20 common allergens and FEV1 were measured as clinical criteria. Three
polymorphisms (G or A at position �C1082, C or T at position �C819 and C or A
at position-592) in the promoter region of the IL-10 gene were genotyped by
polymerase chain reaction and restriction endonuclease digestion.
Results: The level of total
serum IgE was showed significant difference among three genotypes of �C1082
and �C819 polymorphic sites by MIXED procedure analysis (p=0.005 and
p=0.0161 respectively). G allele at �C1082 locus and T allele at �C819 locus
were associated with elevated total serum IgE (p<0.01, OR=3.16;
p<0.05, OR=1.84). None of three IL-10 promoter polymorphisms was
associated with PST and FEV1 phenotypes. There was no significant change in
the allele frequencies of the three loci on the IL-10 gene promoter region
in asthmatic patients with control group. However, we found that the most
common haplotypes such as GTA, GCC, GTA/GCA and ACC/GCC were markedly
different between childhood asthma and controls (c2=8.546,
p<0.05).
Conclusion Polymorphisms within the
IL-10 gene promoter that are associated with elevated total serum IgE may
contribute to the development of the asthmatic phenotype.