0312
THE VALUE OF CVB -RNA ASSAY IN BLOOD
AND THE RELATIONSHIP BETWEEN THE POSITIVE RATE AND COURSE BY PCR TECHNIQUE
TO IDENTIFY VIRAL PATHOGEN OF MYOCARDITIS Ge M1, Ma P-R2, Wang W3, Wang L-Q1,
Wang L2, Li L-W1, Mu C-S1 1 Jinan Railway central Hospital, Jinan, China 2 Shandong Provincial Hospital, Jinan, China 3 Maternity and Child Care Hospital of Shandong
Province, Jinan, China Objective: To realize the value of CVB-RNA assay
in blood by PCR technique to identify viral pathogen of myocarditis and the
relationship between the positive rate and the course. Methods: CVB3 was injected into the abdominal
cavity of 25 mice. Then pathological exam and CVB-RNA assay by PCR
technique was done. CVB-RNA was examed by PCR method in 145 children with
myocarditis.The positive rate of CVB-RNA in myocarditis were compared in
cases whose course within one month and more than one month. Results: 64 of 75 mice (85.3%) had pathological
change as myocarditis. CVB-RNA was positive in 40 of 75 mice (53.3%).The
positive rate of CVB-RNA was 46.2% in the blood of 145 cases with
myocarditis. The positive rate of CVB-RNA in patients whose course shorter
than one month was higher than that of longer than one month. Conclusion: The sensitivity and reliability of
CVB-RNA assay in blood by PCR technigue to identify viral pathogen of
myocarditis. The positive rate was higher in the course of viral
myocarditis patients shorter than one month than those longer than one
month. Key words: Myocarditis, CoxB Virus, PCR technique