0268

APPLICATION OF SDS CHEMICAL METHOD TO EXTRACT OUTER MEMBRANE PROTEINS OF ACINETOBACTER CALCOACETICUS FOR EPIDEMIOLOGIC INVESTIGATION

Zhang Wanming, Wu Shixiao, Liu Guanxin

Hubei Medical Staff College, Jingzhou,.China

Objective: To compare with the results in extracting bacteria outer membrane proteins (OMP) between SDS chemical method and ultrasounic physical method in investigating the epidemiology of A calcoaceticus sepsis in the new-born.

Methods: We used SDS chemical method to break the cell wall of Acinetobacter calcoaceticus for extracting their outer membrane proteins and then performed SDS-PAGE to investigate the epidemiology of A.calcoaceticus sepsis in newborn infants, so did ultrasounic physical method.

Results: SDS chemical method was quite satisfactory. Ultrasounic physical method to break the cell wall of bacteria for OMP extraction needs refigerated ultracentrifuge (147 000xg) which very expensive, while SDS� chemical method only needs high speed centrifuge (38 000xg) which is much cheaper .

Conclusion: SDS chemical method is easy to popularized.