THE CLINICAL STUDY ON PRODUCTION AND mRNA EXPRESSION� OF TGFβ IN CHILDREN NEPHROTIC SYNDROME

Yu Li, Zhuo M-Y, Yang X-S, Zhong Z-M

Department of Pediatrics, Guangzhou First People�s Hospital, Guangzhou, China

Objective: In order to investigate the clinical significance of the transforming growth factorβ(TGFβ) production and mRNA expression in children nephrotic syndrome .

Methods: In 7 boys and 11 girls with primary nephrotic syndrome, TGFβprotein was detected by ELISA method in serum� and� peripheral blood monoeuclear cell(PBMC) cultured� liquid. Endotoxin-induced TGFβmRNA expression in PBMC was measured by reverse transcription polymerase chain reaction (RT-PCR) method. 18 case (13 girls and 5 boys, health children) as control group.

Results: The results were expressed as: (1)TGFβ protein level was higher in children with nephrotic syndrome than control group especially in PBMC cultured� liquid, in serum 33.68�17.31 vs 16.35�3.28, p<0.05; in PBMC cultured� liquid 143.88�12.51 vs 26.24�5.34 p<0.01; (2)TGFβmRNA expression increased in nephrotic syndrome children group, 4.85�1.01 vs 0.04�0.01, p<0.05.

Conclusion: Our findings suggest that the TGFβmRNA expression and TGFβmight play an important role in pathological mechanism of children nephrotic syndrome.

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