0199
Effect of
glycine on endotoxin - induced rat model of necrotizing enterocolitis Liu G-S, Kang J-L, Guan J-B, et al. Objective: Endotoxin can
induce necrotizing enterocolitis (NEC). Tumor necrosis factor-α
(TNF-α) and nitric oxide (NO) play a pivotal role in pathogenesis of
NEC. The amino acid glycine has been shown to protect against endotoxin
shock in the rats by inhibiting TNF-α and NO production. In the
current study we investigated the effect of glycine on endotoxin- induced
rat model of NEC. Methods: In the study group
twenty anesthetized and artificially ventilated rats received 1mg/kg
glycine (20% injection). five minutes later, the rats were treated with
2mg/kg endotoxin (lipopolysaccharide, LPS).in the control group twenty
anesthelized and artificially ventilated rats received only LPS. In all
animals, FiO2 was reduced after 90 min from 0.21 to 0.05 and
ventilation continued until 180 min or death. At the end of the experiment
the samples of blood and intestine were obtained immediately. Serum
TNF-α was measured with ELISA.Serum NO was determined by nitrate
reductase. The histopathology of the necrotic leisions were categoried into
four classes according to the degree of severity: gradeⅠfocal mild injury
confined to villous tips;grade Ⅱ,
partial loss of villi; gradeⅢ,
necrosis extending to submucosa; grade Ⅳ,
transmural necrosis. Result: The survival time
(159.25�22.78 VS
138.75�19.05min)
was shorter in the control group (P<0.01). The intestinal injury of the
study group was markedly improved (P<0.01). The levels of TNF-αand NO in
serum(220.23�90.35 VS
120.7�47.22pg/ml; 211.61�66.37 VS 152.49�64.19μmol/L)
decreased significantly in animals treated with glycine (P<0.01;
P<0.05). Conclusion: Our results of
longer survival time and milder intestinal injury in glycine treated rats
showed that glycine may protect against LPS-induced NEC by inhabiting TNF-αand NO production.
Hospital
Jinan University, Guangzhou, China