0142

An experimental study on the protective mechanism of taurine on murine cardiac muscle cells infected by coxackievirus B3

Wang L¹, Gou XP¹, Ma GF¹, Zhao SM², Cui XD¹.

^{1 Capital Pediatric Institute, Beijing, China}

^{2 PUMCH, Bejing, China}

^{Objective: To provide the basis for taurine (Tau.) therapy for viral myocarditis, the authors studied protective effect of Tau. in murine cardiac cells, mass and serum infected by coxsackievirusB3 (CVB3).}

^{Methods: By using neutral red colorimeter. we observed the survive rates of heart cells and knew the effects of various concentrations of Tau. on cultured normal and those infected by CVB3. After setting models of myocarditis in adult BALB/C mice, mice had been injected 5mM and 20mM Tau for 7 days and killed. By measuring the levels of malondiAldehyde(MDA) within heart muscle and serum, we got the effects of Tau.on whole mice infected by CVB3.}

^{Results: The results showed that the survival rates of cardiac muscle cells increased when cultured in 10mM(P<0.01), 40mM}

^{(P<0.05) and 80mM Tau. fluids and the death rate of cardiac muscle cells infected by CVB3 decreased when cultured in 10mM(P<0.05), 40mM(P<0.05) Tau. fluids. The MDA concentrations in normal myocardial cells and cells infected by CVB3 decreased when 40mM Tau. was added (P<0.05). Also, the levels of MDA in the infected hearts and serum being injected 5mM Tau. were lower than control groups (P<0.05).}

^{Conclusion: The results of this study suggested that Tau could protect cardiac tissue through getting rid of the free redicals in the cells.}